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运用核磁共振波谱技术对临床慢性胰腺炎和胰腺癌患者的胰液样品进行体外波谱学分析,观察和鉴别胰腺癌和慢性胰腺炎的特征代谢物变化,从而为胰腺癌以及相关胰腺疾病的病理研究提供基础数据和实验方法.本文利用胰液样品在室温下能长时间稳定的特性,对其进行了质子的1D、TOCSY和J-resolved等多种NMR实验,并对其主要特征峰进行了指认.实验结果显示在δ1.19的三重峰是这两种病变在胰液中的主要区别:只在慢性胰腺炎图谱中观察到,而在胰腺癌图谱中未见.2D实验结果表明此特征峰为乙氧基中的甲基峰,而乙氧基可能与胰腺中乙醇的代谢有关.
In vitro spectroscopic analysis of pancreatic juice samples from patients with chronic pancreatitis and pancreatic cancer using nuclear magnetic resonance spectroscopy was performed to observe and identify the characteristic metabolites of pancreatic cancer and chronic pancreatitis so as to provide the pathological study of pancreatic cancer and related pancreatic diseases Basic data and experimental methods.In this paper, many kinds of NMR experiments such as proton 1D, TOCSY and J-resolved proton were carried out using long-term stability of pancreatic juice samples at room temperature, and the main characteristic peaks were identified. The results showed that the triplet peak at δ1.19 was the major difference between the two lesions in pancreatic juice: only in chronic pancreatitis but not in pancreatic cancer.2D experimental results indicate that this characteristic peak is ethoxy Methyl group in the base, and ethoxy may be related to the metabolism of ethanol in the pancreas.