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目的:观察二甲双胍联合辛伐他汀对高糖诱导肾小球系膜细胞增殖的抑制作用。方法:采用肾小球系膜细胞体外培养技术,筛选最佳葡萄糖浓度,在此浓度下,诱导肾小球系膜细胞增殖,在不同浓度二甲双胍联合辛伐他汀浓度,通过MTT法检测并观察不同时间,不同浓度下系膜细胞增殖情况。结果:与其他剂量比较,0.6 g·L~(-1)葡萄糖在48、72 h对肾小球系膜细胞增殖刺激作用较强,差异有统计学意义(P<0.01),选0.6 g·L~(-1)葡萄糖为最佳浓度。与高糖组比较,二甲双胍联合辛伐他汀高、中及低剂量组,均在48、72 h抑制肾小球系膜细胞生长(P<0.05);其中二甲双胍联合辛伐他汀高剂量组的抑制较强,但与二甲双胍联合辛伐他汀中剂量组、低剂量组比较,差异不显著(P>0.05)。结论:二甲双胍联合辛伐他汀具有抑制肾小球系膜细胞增殖作用,联合用药可治疗糖尿病肾病。
Objective: To observe the inhibitory effect of metformin combined with simvastatin on the proliferation of mesangial cells induced by high glucose. Methods: Glomerular mesangial cells cultured in vitro technology, screening the best glucose concentration, in this concentration, mesangial cell proliferation was induced in different concentrations of metformin combined with simvastatin concentrations by MTT assay and observed different Time, different concentrations of mesangial cell proliferation. Results: Compared with other doses, 0.6g · L -1 glucose stimulated the proliferation of glomerular mesangial cells at 48 and 72 h, with a significant difference (P <0.01) L ~ (-1) glucose is the best concentration. Compared with high glucose group, metformin combined with simvastatin in high, medium and low dose groups inhibited the growth of mesangial cells 48 and 72 h (P <0.05), and metformin combined with simvastatin high dose group inhibited But no significant difference compared with the middle dose of simvastatin combined with metformin and the low dose of simvastatin (P> 0.05). Conclusion: Metformin combined with simvastatin can inhibit the proliferation of glomerular mesangial cells and the combination therapy can treat diabetic nephropathy.