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目的研究胶原诱导性关节炎(CIA)大鼠不同时期血清蛋白表达谱,寻找与疾病发生和炎症相关的特异表达蛋白,初步探讨类风湿关节炎(RA)的发病机制。方法以含有19个细胞因子的抗体芯片分别检测CIA大鼠发病高峰期和后期及正常大鼠的血清蛋白表达谱,并以酶联免疫吸附试验法(ELISA)测定血清肿瘤坏死因子(TNF)-α表达,验证微阵列检测结果。结果CIA高峰期和后期持续显著上调的细胞因子有10个,高峰期特异高表达细胞因子有1个。微阵列结果没有显示显著下调的细胞因子。结论CIA疾病不同时期细胞因子表达谱不同,筛选出的特异表达蛋白不仅为进一步研究RA的病理机制提供线索,也为疾病的早期诊断提供分子标志。
Objective To investigate the expression profile of serum proteins in different stages of collagen-induced arthritis (CIA) in rats and to find the specific expression proteins related to the pathogenesis and inflammation of the rats, and to explore the pathogenesis of rheumatoid arthritis (RA). Methods Serum protein expression profiles were detected by antibody chip containing 19 cytokines at the peak and the late phase of CIA rats and normal rats respectively. Serum tumor necrosis factor (TNF) - α expression, verify the microarray test results. Results There were 10 cytokines that were continuously and significantly up-regulated in the peak and the late period of CIA, and 1 in the peak-specific high expression of cytokines. Microarray results showed no significant downregulation of cytokines. Conclusions The expressions of cytokines in different stages of CIA are different. The screened proteins not only provide clues for further studying the pathological mechanism of RA, but also provide molecular markers for the early diagnosis of the disease.