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目的:探讨Fas与Fas L在芪白平肺胶囊含药血清诱导低氧大鼠肺动脉平滑肌细胞(pulmonary arterysmooth muscle cells,PASMCs)凋亡中的作用。方法:芪白平肺胶囊含药血清和空白血清的制备。大鼠PASMCs的培养及a-SMA细胞免疫荧光鉴定。MTT法筛选最佳含药血清及最佳低氧时间。正式实验根据MTT结果分为常氧对照组,低氧模型组,最佳含药血清组,含药血清作用最佳低氧时间后,TUNEL-FITC/DAPI法检测各组PASMCs凋亡情况,Western Blot法检测各组PASMCs的Fas与Fas L蛋白表达变化。结果:与低氧模型组比较,芪白平肺胶囊含药血清可明显促进低氧大鼠PASMCs的凋亡,但细胞内Fas与Fas L蛋白表达较模型组无显著差异。结论:芪白平肺胶囊含药血清能有效抑制低氧大鼠PASMCs增殖作用,促进其凋亡,但Fas/Fas L死亡受体通路可能不参与芪白平肺胶囊含药血清诱导低氧大鼠PASMCs凋亡的调控。
AIM: To investigate the role of Fas and Fas L in the apoptosis of pulmonary arterial muscle cells (PASMCs) induced by hypoxia in Qibai Pingfei capsule-containing serum. Methods: Qibai Pingfei capsule containing serum and blank serum preparation. Culture of rat PASMCs and identification of a-SMA cells by immunofluorescence. MTT screening of the best drug-containing serum and hypoxia best time. According to MTT results, formal experiments were divided into normoxia control group, hypoxia model group, best drug-containing serum group, and drug-containing serum for best hypoxia time. TUNEL-FITC / DAPI method was used to detect the apoptosis of PASMCs in each group. Blot method was used to detect the expression of Fas and Fas L protein in PASMCs in each group. Results: Compared with the hypoxia model group, Qibai Pingfei capsule-containing serum could obviously promote the apoptosis of PASMCs in hypoxic rats, but there was no significant difference in the expression of Fas and Fas L protein among the model groups. Conclusion: Qibai Pingfei capsule containing serum can effectively inhibit the proliferation of PASMCs in hypoxic rats and promote their apoptosis, but Fas / Fas ligand pathway may not participate in the Qibai Pingfei capsule containing serum induced hypoxia Regulation of apoptosis in murine PASMCs.