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采用凝胶色谱法，以２０６ｎｍ处的紫外吸收为检测手段，分别从尿毒症患者血 清及正常人尿液内分离出Ａ，Ｂ两个中分子峰，而从正常人血清中分离出来的Ａ峰远低 于尿毒症患者血清的Ａ峰；在Ｂ峰的位置正常人血清未出现明显的吸收．运用该方法 不仅提高了凝胶色谱的分离效果，且能方便地得到中分子级分的纯品．对清除了淋洗 液组分的中分子级分进行紫外和红外扫描，结果发现来源于尿毒症患者血清及正常人 尿液的Ａ峰物质具有相同的紫外吸收且红外吸收光谱极其相似，而不同来源的Ｂ峰物 质虽然紫外吸收相同，但它们的红外吸收却存在一定的差异．采用离子交换色谱法对Ｂ 峰物质进行进一步的分离，以２３０ｎｍ处的紫外吸收为检测手段，正常人尿液的Ｂ峰物 质被分离成１７个亚峰，尿毒症血清的Ｂ峰物质被分离成１３个亚峰．其中绝大部分亚峰 的出峰位置（洗脱体积）相同．
Gel chromatography was used to detect the UV absorbance at 206 nm as the detection method. Two A and B middle molecular peaks were separated from the urine of patients with uremia and normal urine, respectively, while the peak A was separated from normal human serum Far lower than the serum A peak of uremia patients; no significant absorption of normal human serum at peak B position. The method not only improves the separation effect of gel chromatography, but also can easily obtain the pure product of the middle molecular fraction. UV-visible and infrared-based scans of the middle molecular fraction with the eluent fraction removed revealed that the A-peaked material from the urine of patients with uremia and normal urine had the same UV absorbance and the IR spectra were very similar and different Although the source of the B peak substances have the same ultraviolet absorption, there are some differences in their infrared absorption. B ion exchange chromatography was used to further separate the B peak to detect the UV absorption at 230 nm. The B peak of normal urine was separated into 17 sub peaks, and the B peak of uremic serum was separated into 13 Asian peak. Most of the sub-peak peak position (elution volume) the same.