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Wnt信号参与了小鼠早期神经发育。我们以往的实验结果表明，Wnt信号可引起P19胚胎性癌细胞的神经分化。为了进一步了解Wnt信号在P19神经分化过程中行使功能的时间，我们以Wnt信号通路关键成员β-catenin是否定位在细胞核中作为考察Wnt信号是否能传递到细胞核内调控下游基因活性的指标，分析了Wnt信号在P19细胞神经分化过程中的活性。我们观察到，β-catenin在RA诱导的P19细胞分化的第2到第4天存在核定位。在此期间，Wnt下游基因En－2的表达量也有明显增加。因此，在P19神经分化过程中，Wnt活性可能发生在这一阶段。同时，在神经突的形成、伸长及神经纤维集束过程中，β-catenin在神经突起及神经纤维上也存在特异性分布，提示在此过程中β-atenin可能也行使了功能。
Wnt signaling is involved in mouse early neurodevelopment. Our previous experimental results show that Wnt signaling can cause neural differentiation of P19 embryonal cancer cells. To further understand the function of Wnt signaling during P19 neural differentiation, we analyzed whether the Wnt signalling key member β-catenin is located in the nucleus as an index to examine whether Wnt signaling can be transmitted to the nucleus and regulate downstream gene activity. The activity of Wnt signaling during neural differentiation of P19 cells. We observed that there was a nuclear localization of β-catenin on the 2nd to 4th days of RA-induced P19 cell differentiation. During this period, the expression of En-2, a downstream gene of Wnt, also increased significantly. Therefore, Wnt activity may occur at this stage during P19 neural differentiation. At the same time, β-catenin also has a specific distribution in neurites and nerve fibers during neurite formation, elongation, and nerve fiber bundles, suggesting that β-atenin may also function during this process.