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基于先期抽薹敏感的‘松滋野生’和耐抽薹的‘Amsterdam’胡萝卜转录组测序结果,分析胡萝卜中SOC1同源基因对光周期的响应规律及其功能。胡萝卜中存在2个SOC1同源基因:DcSOC-1和DcSOC-2,具有完整的开放阅读框(ORF),分别编码217和211个氨基酸,均包含MADS-box和K-box结构域,C末端含有保守的SOC1 motif基序。系统进化树分析表明,DcSOC-1和DcSOC-2与猕猴桃Ac SOC1f和葡萄Vv SOC1的亲缘关系较近,属于SOC1/TM3亚家族。在长/短日照处理下,DcSOC-1在‘松滋野生’叶片中的相对表达量均显著高于‘Amsterdam’,且长日照能促进其表达,而DcSOC-2在‘松滋野生’和‘Amsterdam’中的表达水平较低。在春、秋两季生长过程中,DcSOC-1在‘松滋野生’叶片中的最高表达量分别达到522.4和401.7,而在‘Amsterdam’中均呈低水平表达,与先期抽薹率呈极显著正相关,说明DcSOC-1对胡萝卜的先期抽薹起着重要作用。
Based on the early bolting-sensitive ’Songzi wild’ and bolting-resistant ’Amsterdam’ carrot transcriptome sequencing results, the response rule and function of SOC1 homologue in photoperiod of carrot were analyzed. Two SOC1 homologous genes, DcSOC-1 and DcSOC-2, exist in carrot and have a complete open reading frame (ORF) encoding 217 and 211 amino acids, respectively, and contain the MADS-box and K-box domains. The C-terminal Contains a conserved SOC1 motif motif. Phylogenetic tree analysis showed that DcSOC-1 and DcSOC-2 were closely related to Act1 Kiwi and Vv SOC1, belonging to the SOC1 / TM3 subfamily. The relative expression level of DcSOC-1 in the leaves of ’Songzi wild’ was significantly higher than that of ’Amsterdam’ under long / short daylight treatment and the expression of DcSOC-2 was promoted by long-day sunshine while that of DcSOC- The expression level in ’Amsterdam’ is low. In spring and autumn, the highest expression level of DcSOC-1 was 522.4 and 401.7 in the leaves of ’Songzi Wild’, respectively, while it was low in ’Amsterdam’, which was significantly different from the early bolting rate Positive correlation indicates that DcSOC-1 plays an important role in the early bolting of carrots.