本文报告了精原细胞法用于筛选抗肿瘤植物粗制剂的条件和筛选结果。初筛剂量取100～1000mg/ml 生药。阳性指标为两个以上曲精管第7～8相精原细胞明显减少,而其它造精细胞无改变,或同时伴有精母细胞减少或伴有精子细胞变性,结果可重复者。精原细胞法和动物体内抗瘤作用有较好的相关性,敏感,重复性好,可用于植物粗制剂的初筛。 This article reports the conditions and screening results for the screening of crude preparations of anti-tumor plants using spermatogonia. The initial screening dose is 100 to 1000 mg/ml crude drug. The positive indicator was that the number of spermatogonia in the 7th to 8th phase of two or more seminiferous tubules was significantly reduced, while other spermatogenic cells were not changed, or spermatogonia was reduced or accompanied by sperm cell degeneration. The results were repeatable. Spermatogonia and animal anti-tumor effects in vivo have a good correlation, sensitive and reproducible, can be used for primary screening of plant crude preparations.