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目的:检测缺氧诱导因子-1α(HIF-1α)在鼻咽癌和慢性鼻咽炎组织中的表达,并用CD34标记测定微血管密度(MVD),探讨HIF-1α和MVD在鼻咽癌各种临床病理因素中的作用以及它们之间的相互联系。方法:采用免疫组织化学SABC及SP法检测58例鼻咽癌和20例慢性鼻咽炎组织中HIF-1α的表达情况,并用CD34行免疫组织化学染色,计数MVD,并结合临床病理特征进行分析。结果:鼻咽癌和慢性鼻咽炎组织HIF-1α的阳性表达率分别为79.31%和10.00%,差异具有统计学意义(P<0.01)。鼻咽癌组织MVD为39.32±7.99,慢性鼻咽炎为18.73±2.39,差异有统计学意义(P<0.01)。鼻咽癌组织中,有淋巴结转移组HIF-1α阳性表达率(92.6%)明显高于无淋巴结转移组(67.7%),有淋巴结转移组MVD(44.41±5.63)高于无淋巴结转移组(34.89±7.09)。不同临床病理分期的HIF-1α的表达和MVD均差异有统计学意义(P<0.05或P<0.01)。HIF-1α的表达与MVD呈正相关(P<0.01)。结论:HIF-1α参与了鼻咽癌的发生、浸润和转移;血管生成在鼻咽癌的浸润和转移中起了重要作用;鼻咽癌中HIF-1α的表达与肿瘤血管生成密切相关,在鼻咽癌发展过程中起重要作用,可能成为抗肿瘤治疗的潜在靶点。
OBJECTIVE: To detect the expression of hypoxia-inducible factor-1α (HIF-1α) in nasopharyngeal carcinoma and chronic nasopharyngitis and to determine the microvessel density (MVD) by CD34 labeling. To investigate the expression of HIF-1α and MVD in nasopharyngeal carcinoma The role of pathological factors and their interrelationships. Methods: The expressions of HIF-1α in 58 cases of nasopharyngeal carcinoma and 20 cases of chronic nasopharyngitis were detected by immunohistochemical SABC and SP method. The expression of HIF-1α was detected by immunohistochemical staining and MVD in CD34, and the clinicopathological features were analyzed. Results: The positive rates of HIF-1α in nasopharyngeal carcinoma and chronic nasopharyngitis tissues were 79.31% and 10.00%, respectively, with statistical significance (P <0.01). The MVD of nasopharyngeal carcinoma was 39.32 ± 7.99 and that of chronic nasopharyngitis was 18.73 ± 2.39, the difference was statistically significant (P <0.01). The positive rate of HIF-1α expression in NPC (92.6%) was significantly higher than that in non-lymph node metastasis (67.7%), and the MVD in lymph node metastasis (44.41 ± 5.63) was higher than that in non-lymph node metastasis ± 7.09). There was significant difference in the expression of HIF-1α and MVD in different clinicopathological stages (P <0.05 or P <0.01). The expression of HIF-1α was positively correlated with MVD (P <0.01). Conclusions: HIF-1αis involved in the occurrence, invasion and metastasis of nasopharyngeal carcinoma. Angiogenesis plays an important role in the invasion and metastasis of nasopharyngeal carcinoma. The expression of HIF-1α in nasopharyngeal carcinoma is closely related to tumor angiogenesis. Nasopharyngeal carcinoma plays an important role in the process of development and may become a potential target for anti-tumor therapy.