论文部分内容阅读
睡眠剥夺是一种能够实现个体睡眠部分或完全丧失的技术手段,由睡眠干扰所引发的逐渐积累的睡眠压力能导致多种生理方面的变化,甚至是个体的死亡.在本次研究中,我们使用旋转滚动式睡眠剥夺仪对大鼠进行了长达14天的睡眠剥夺,同时我们制作了一种16通道的微电极阵列并将其植入到大鼠海马区进行实时电生理信号检测.结果显示睡眠剥夺之后,大鼠海马区内的椎体神经元和中间神经元动作电位幅值提升,两种神经元动作电位的发放频率也显著增大.同时,场电位的波动也更加剧烈.神经细胞在睡眠剥夺后的快速发放模式表明长期清醒状态下神经细胞兴奋性的提升.此外,场电位在0-50 Hz频段的平均功率计算结果显示,睡眠剥夺之后各个频段的功率均有所提升,且在δ频段的变化最为明显.场电位在低频段的功率改变表明了睡眠剥夺所致的睡眠压力增大,此改变还将会进一步损伤大脑的相关功能.“,”Sleep deprivation(SD)is the partial or complete loss of sleep and has long been used as a tool in sleep research to interfere with normal sleep cycles in rodents and humans.The progressively-accumulating sleep pressure induced by sleep deprivation can lead to a variety of physiological changes and even death.Compared to traditional detection methods,in vivo detection of neuronal activity using micro-electromechanical system(MEMS)technology following sleep deprivation can help fully elucidate the effects of sleep deprivation at the cellular level.Herein,a computer-controlled rotary roller was used to completely deprive rats of sleep for 14 days and 16-channel microelectrode arrays(MEAs)were fabricated and implanted into the rat hippocampus to measure neural spikes and local field potentials(LFPs)in real-time.The hippocampus is involved in learning and memory and has been the focus of intensive research aimed at understanding the function of sleep.This study was performed to measure the changes in neuronal activity in the rat hippocampus induced by sleep deprivation as well as their overall impact on the brain.After sleep deprivation,both the pyramidal-and inter-neurons showed a higher amplitude and more intense firing patterns.The fast-firing pattern of the neurons after sleep deprivation indicated elevated excitability in the prolonged awake state.In addition,the LFP of the sleep deprived rats fluctuated more frequently.The power of the LFPs in the low-frequency band(0-50 Hz)was calculated,showing increased power of the delta,theta,alpha,and beta bands after sleep deprivation,especially for the delta band(0.1-4 Hz).Generally,LFPs are generated by all types of neural activity in the neural circuit,and the changes in the low frequency band power suggested decreased arousal and increased sleep pressure induced by sleep deprivation,which could further impair brain function.This study was mainly aimed at measuring electrophysiological changes induced by sleep deprivation in the rat brain.Typically,neuronal activity changes were accompanied by the alternation of specific neurotransmitters in the brain.In the future,it will be essential to focus on measuring the concurrent change of electrophysiological and neurochemical signals to better examine the impact of sleep deprivation on brain function.