直接扩增法可以免去提取的繁琐步骤,采用商品化直扩试剂盒,省时省力,能有效降低成本,因而被广泛用于DNA数据库建设。笔者对本实验室1年内受理案件的血斑检材采用直接扩增(简称直扩法)和经过磁珠法提取后再进行扩增(简称磁珠法)两种方法检验,比较两种方法对血斑类检材的检验效果,以期为相关检验提供参考。 Direct amplification method can eliminate the cumbersome extraction steps, using commercial DSS kit, saving time and labor, can effectively reduce costs, which has been widely used in DNA database construction. The author of the laboratory within 1 year to accept the case of blood spots detected by direct amplification (referred to as direct expansion method) and after the magnetic beads method to extract and then amplified (referred to as magnetic beads method) two methods of testing, comparing the two methods Blood spots of the inspection results, with a view to provide relevant reference for the test.