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目的对重庆市流行的艾滋病(AIDS)病毒1型(HIV-1)进行分子流行病学调查,为艾滋病预防控制策略的制定提供依据。方法用套式聚合酶链反应(nested-PCR)对31份重庆市HIV-1抗体阳性者外周血单个核细胞(PBMC)中前病毒DNA的膜蛋白基因的C2-V3区,及其邻区300个核苷酸序列进行测定和分析。结果31份标本中有21份扩增阳性并得到相应序列,扩增率为67.6%。经过基因离散率计算和系统树分析后证实:1份标本为CRF01-AE流行重组毒株,20份标本为HIV-1 BC重组毒株。结论目前重庆市HIV-1 BC重组病毒为优势流行株,为有效控制HIV流行,需要重点在吸毒人群中开展行为干预措施。
Objective To investigate the molecular epidemiology of HIV-1, which is endemic in Chongqing, and provide basis for the development of AIDS prevention and control strategies. Methods The C2-V3 region of the proviral DNA in peripheral blood mononuclear cells (PBMCs) of 31 HIV-1 positive individuals in Chongqing were detected by nested-PCR and their adjacent regions 300 nucleotide sequences were determined and analyzed. Results 21 out of 31 specimens were positive for amplification and got the corresponding sequence with an amplification rate of 67.6%. After genetic distance calculation and phylogenetic tree analysis confirmed: 1 copy of the CRF01-AE epidemic strains, 20 copies of the HIV-1 BC recombinant strain. Conclusion Currently, Chongqing HIV-1 BC recombinant virus is the predominant epidemic strain. In order to effectively control the epidemic of HIV, behavioral interventions should be focused on drug users.