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背景:溃疡病患者常出现各种细胞免疫和体液免疫障碍。目的:探讨细胞因子白细胞介素（IL）-10、IL-12和干扰素（IFN）-γ在十二指肠球部溃疡（DU）发生中的作用。方法:在奥美拉唑、克拉霉素、替硝唑一周三联疗法治疗之前和之后4～6周从DU患者的球部钳取轴膜组织,应用酶联免疫吸附测定（ELISA）检测IL-10、IL-12和IFN-γ的含量,并对照组进行比较。结果:一周三联疗法治疗前,DU患者球部劾膜的IL-10、IL-12和IFN-γ含量（17.983pg/ml±8.676pg/ml、44098 pg/ml±12.842 pg/ml±和82.373pg/ml±17.264pg/ml±）均显著高于对照组（85.99 pg/ml±4.147 pg/ml、17.550 pg/ml±5.980 pg/ml和26.298pg/ml±10.385 pg/ml,p<0.01）,且IL-10与IL-12的含量呈正相关（r=0.87,P<0.01）,IL-10与IFN-γ的含量也呈正相关（r=0.92,P<0.01）。治疗后,DU患者球部轴膜的IL-10IFN-γ含量均有所下降（17.289 pg/ml±3.939 pg/ml和27.030 pg/ml±3.753 pg/ml,P<0.01）,但IL-10的含量仍持续维持在较高水平（26.098 pg/ml±15.152 pg/ml,P<0.01）。结论:细胞因子IL-10、IL-12和IFN-γ可能在DU的发生机制中起重要作用。一周三联疗法治疗后,促炎症细胞因子IL-12和IFN-γ分泌得到有效抑制,而抗炎症细胞因子IL-10持续维待在较高水平,为黏?
Background: A variety of cellular and humoral immune disorders occur in patients with ulcer disease. Objective: To investigate the role of interleukin (IL) -10, IL-12 and interferon-γ (IL-12) in the pathogenesis of duodenal ulcer (DU). Methods: Axial membrane was harvested from the ball of DU patients before and after treatment with omeprazole, clarithromycin, and tinidazole for one week. ELISA was used to detect the expression of IL- 10, IL-12 and IFN-γ, and the control group were compared. Results: The levels of IL-10, IL-12 and IFN-γ in synovium of DU patients before treatment with triple-combined therapy for one week (17.983pg / ml ± 8.676pg / ml, 44098pg / ml ± 12.842pg / ml ± and 82.373 pg / ml ± 17.264 pg / ml ±) were significantly higher than those of the control group (85.99 pg / ml ± 4.147 pg / ml, 17.550 pg / ml ± 5.980 pg / ml and 26.298 pg / ml ± 10.385 pg / (R = 0.87, P <0.01). The content of IL-10 and IFN-γ also showed a positive correlation (r = 0.92, P <0.01). After treatment, the IL-10IFN-γconcentration in the ball of the bulb decreased in DU patients (17.289 pg / ml ± 3.939 pg / ml and 27.030 pg / ml ± 3.753 pg / ml, P <0.01) (26.098 pg / ml ± 15.152 pg / ml, P <0.01). Conclusion: The cytokines IL-10, IL-12 and IFN-γ may play an important role in the pathogenesis of DU. After a week of triple therapy, proinflammatory cytokines IL-12 and IFN-γ secretion was effectively inhibited, while the anti-inflammatory cytokines IL-10 sustained at a higher level for the sticky?