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目的 筛查中国 5地区幽门螺杆菌 (Hp)插入序列IS6 0 5、IS6 0 6的分布。方法 选取来自北京、福建、云南、沈阳、香港等地共 10 4株Hp ,用PCR及染色体打点杂交的方法筛查IS6 0 5、IS6 0 6的分布 ,并对其分布特点进行分析。结果 中国 5地区Hp菌株IS6 0 5的阳性率为 4 0 % ,IS6 0 6的阳性率为 18%。云南菌株IS6 0 5的阳性率有高于其他地区的趋势 ,不同地区IS6 0 6的分布差异无显著性。不同临床诊断结果分离的菌株IS6 0 5、IS6 0 6的分布差异无显著性。IS6 0 5的两个开码读框ORFA、ORFB共存。结论 中国 5地区Hp菌株广泛存在IS6 0 5、IS6 0 6 ,且IS6 0 5的分布可能与菌株分离个体的地理位置有关。
Objective To screen the distribution of Hp insertion sequences IS6 0 5 and IS6 0 in China 5 regions. Methods A total of 104 Hp strains from Beijing, Fujian, Yunnan, Shenyang and Hong Kong were selected. The distribution of IS6 0 5 and IS6 0 6 was screened by PCR and chromosomal dot blot hybridization. The distribution characteristics were analyzed. Results The prevalence of Hp strain IS6 0 5 in China was 40% and that of IS6 0 6 was 18%. The positive rate of Yunnan strain IS6 0 5 was higher than that of other regions, and the distribution of IS6 0 6 in different regions was not significantly different. There was no significant difference in the distribution of IS6 0 5 and IS6 0 6 isolated from different clinical diagnosis results. IS6 0 5 open reading frame ORFA, ORFB coexistence. Conclusions There are widespread IS6 0 5 and IS6 0 6 strains of Hp strains in China’s 5 regions, and the distribution of IS6 0 5 may be related to the geographic location of individual isolates from strains.