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Dexter等报告的长期骨髓培养系统,可维持造血干细胞(CFU-S)和粒-巨噬祖细胞的增殖达数月。在此培养系统中,第一次种入骨髓细胞形成贴壁的单层,具有造血诱导微环境的功能,它能为第二次种入的干细胞在体外增殖提供场所。但是,有报道认为,在此系统中生长的不仅有来自第二次种入的骨髓CFU—S,而且也含有第一次种入的CFU—S,它亦能在其中增殖。本研究致力于寻找一种X线照射的最适剂量,借以保存贴壁细胞的功能而清除第一次种入的
The long-term bone marrow culture system reported by Dexter et al. Maintains the proliferation of hematopoietic stem cells (CFU-S) and granulo-macrophage progenitor cells for several months. In this culture system, the first time into the bone marrow cells to form an adherent monolayer, has the function of hematopoietic microenvironment, it can provide a place for the second in vitro stem cell proliferation in vitro. However, it has been reported that not only the second bone marrow CFU-S but also the first CFU-S that is grown in this system can also grow therein. This study sought to find an optimal dose of X-ray irradiation in order to preserve the function of adherent cells while removing the first implant