目的探讨细粒棘球蚴生发细胞原代培养的改进方法。方法剪碎自然感染的绵羊棘球蚴包囊组织,用胶原酶与胰蛋白酶等比混合液消化解离,对获得的细胞以显微镜、电镜以及免疫荧光试验方法进行形态学研究及鉴定。结果分离出足量细胞进行体外培养且细胞具有生发细胞样形态和免疫特征。结论改良的胶原酶消化法是一种简单有效的生发细胞分离培养方法 。 Objective To investigate the improved method of primary culture of germinated cells of Echinococcus granulosus. Methods Echinococcus oocysts were dissected naturally, and the cells were digested and dissociated by collagenase and trypsin. The obtained cells were studied by morphology, microscopy, electron microscopy and immunofluorescence. As a result, a sufficient amount of cells were isolated for in vitro culture and the cells had germinal cell-like morphology and immune characteristics. Conclusion Improved collagenase digestion method is a simple and effective germinal cell culture method.