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向Wistar大鼠脑内微量注射6羟多巴胺(6-OHDA)破坏一侧黑质,造成Apomorphine诱发的旋转模型。将同系大鼠肾上腺髓质植入损伤侧尾状核,两个月后观察行为改变。其中4/10停止旋转,6/10旋转次数明显减少。用高效液相电化学检测器(HPLC-ED)测损伤侧端脑多巴胺(DA)含量,发现比健侧减少37％(P<0.01),其代谢产物高香草酸(HVA)也明显减少(P<0.05)。经肾上腺髓质移植后,DA含量仅增长9％,代谢产物无变化,去甲肾上腺素(NE)含量明显增高(P<0.05)。此外还发现损伤侧脑突触体对DA的摄取明显增加,DA_2受体结合活性显著增高(P<0.01),这些变化在脑移植大鼠组未能测到。
Microinjection of 6-hydroxydopamine (6-OHDA) into the brain of Wistar rats disrupted one side of the substantia nigra, resulting in a spin-induced model of Apomorphine. Adrenal medulla was implanted into injured caudate nucleus of cauda equina, and behavioral changes were observed after two months. Among them, 4/10 stopped rotating and the number of 6/10 rotation decreased significantly. The content of dopamine (DA) on the injured side was measured by HPLC-ED and found to be 37% less than that of the contralateral side (P <0.01) and its metabolite homovanillic acid (HVA) P <0.05). After transplanting adrenomedullin, the content of DA increased only 9%, the metabolites did not change and the content of norepinephrine (NE) was significantly increased (P <0.05). In addition, it was found that DA increased uptake and DA2 receptor binding activity (P <0.01), which were not detected in brain transplantation rats.