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目的通过研究高效氯氟氰菊酯对德国小蠊超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)2种保护酶活性的影响,进一步探讨高效氯氟氰菊酯的作用机制,为德国小蠊的防治工作提供理论基础和方法参考。方法参照国标GB13917.1-13917.8-92《农药登记卫生杀虫剂室内药效试验方法》喷施一定剂量不同浓度的高效氯氟氰菊酯,用酶活性测定试剂盒测定德国小蠊体内2种保护酶活性的时间变化。结果不同浓度的高效氯氟氰菊酯对德国小蠊SOD和GPX的诱导作用存在时效上的不同,诱导强度与高效氯氟氰菊酯的浓度呈一定的正相关关系,144h内SOD和GPX的酶活性均呈现“先升高—后降低—再升高”的趋势。结论低致死浓度高效氯氟氰菊酯对德国小蠊SOD和GPX有明显的诱导作用,SOD和GPX活性的升高与德国小蠊对高效氯氟氰菊酯等拟除虫菊酯类杀虫剂的抗药性及耐药性增强有关。
Objective To investigate the effects of lambda-cyhalothrin on the activities of two protective enzymes of Blattella germanica, superoxide dismutase (SOD) and glutathione peroxidase (GPX), and further investigate the effects of lambda-cyhalothrin Mechanism of action for the prevention and treatment of Blattella to provide the theoretical basis and method reference. Methods Reference to national standard GB13917.1-13917.8-92 “Pesticides registered health insecticides indoor efficacy test method” sprayed with a certain dose of different concentrations of cyhalothrin, with the enzyme activity determination kit German Cockroach in vivo 2 Changes in the protective enzyme activity over time. Results Different concentrations of lambda-cyhalothrin showed different aging effects on the SOD and GPX of Blattella germanica. The correlation between the induction intensity and the concentration of lambda-cyhalothrin was positive. The SOD and GPX Enzyme activity showed the trend of “first increase - later decrease - then increase”. CONCLUSION: The low lethal concentration of lambda-cyhalothrin can induce the SOD and GPX of Blattella germanica obviously. The increase of the activities of SOD and GPX is closely related to the increase of the activity of pyronaridine insecticides such as lambda-cyhalothrin Resistance and enhanced resistance related.