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目的探讨抑癌基因FRK(Fyn-related kinase)影响胶质瘤细胞侵袭和迁移的机制。方法将真核表达质粒pcDNA3.0-FRK和对照质粒pcDNA3.0转入人胶质瘤U25l细胞中,western blot技术检测FRK/N-cadherin/E-cadherin蛋白表达,细胞划痕试验检测细胞迁移能力,Transwell侵袭实验检测细胞侵袭能力。结果与对照组比较,转染pcDNA3.0-FRK质粒24 h后U25l细胞侵袭能力下降47%、迁移能力下降64%,差异均有统计学意义(P<0.01);转染pcDNA3.0-FRK可以明显增加N-cadherin/E-cadherin的表达。结论FRK可以通过增加N-cadherin/E-cadherin的表达,进而抑制胶质瘤细胞侵袭和迁移能力。
Objective To investigate the mechanism of tumor suppressor gene FRK (Fyn-related kinase) affecting the invasion and migration of glioma cells. Methods The eukaryotic expression plasmid pcDNA3.0-FRK and the control plasmid pcDNA3.0 were transfected into human glioma U251 cells. The protein expression of FRK / N-cadherin / E-cadherin was detected by western blot and cell migration assay Ability, Transwell invasion assay to detect cell invasion ability. Results Compared with the control group, the invasive ability of U251 cells decreased by 47% and the migration ability decreased by 64% after transfected with pcDNA3.0-FRK plasmid for 24 h, the difference was statistically significant (P <0.01); transfected with pcDNA3.0-FRK Can significantly increase the expression of N-cadherin / E-cadherin. Conclusion FRK can inhibit the invasion and migration of glioma cells by increasing the expression of N-cadherin / E-cadherin.