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目的:考察莱菔子水提液中萝卜苷在大鼠小肠的吸收特性。方法:采用大鼠在体单向肠灌流法考察不同药物质量浓度(0.02,0.05,0.1 g·mL-1)对萝卜苷肠吸收特性的影响,计算吸收速率常数(Ka)和表观吸收系数(Papp)。采用HPLC测定萝卜苷含量,流动相0.1%磷酸溶液-乙腈(87∶13),检测波长225 nm。结果:萝卜苷在pH 5.12的K-R试液中稳定性最好。不同质量浓度的莱菔子水提液中萝卜苷在大鼠小肠的Ka分别为(7.750±1.744)×10-3,(7.204±1.045)×10-3,(7.148±0.837)×10-3min-1,Papp分别为(1.519±0.507)×10-3,(1.504±0.617)×10-3,(1.592±0.345)×10-3cm·min-1,均无显著性变化。结论:萝卜苷为易吸收成分,在大鼠小肠中的吸收机制为被动扩散。
OBJECTIVE: To investigate the absorption characteristics of glucoraphenin in the small intestine of Radish extracts. Methods: The intestine absorption characteristics of glucoraphanin in different concentrations (0.02,0.05 and 0.1 g · mL-1) of rats were investigated by in vivo one-way intestinal perfusion in rats. The absorption rate constant (Ka) and apparent absorption coefficient (Papp). The content of glucoraphanin was determined by HPLC. The mobile phase consisted of 0.1% phosphoric acid solution - acetonitrile (87:13) and the detection wavelength was 225 nm. Results: The best stability of glucosinolates in K-R solution at pH 5.12. The Ka of the glucoraphenin in the different concentrations of Radish extracts was (7.750 ± 1.744) × 10-3, (7.204 ± 1.045) × 10-3, (7.148 ± 0.837) × 10-3min- 1 and Papp were (1.519 ± 0.507) × 10-3, (1.504 ± 0.617) × 10-3, (1.592 ± 0.345) × 10-3cm · min-1, respectively. No significant changes were found. Conclusion: Glucoraphenin is an easily absorbed component and its absorption mechanism in the small intestine of rats is passive diffusion.