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目的：对６种四甲基哌啶氮氧自由基及衍生物（４ＴＥＭＰＯ）抗脂质过氧化作用进行了实验研究，为其进一步研究及应用于临床作为抗自由基损伤药物提供依据。方法：用Ｆｅ２＋ＶｉｔＣ引发大鼠肝细胞膜、心肌细胞膜、肝线粒体的脂质过氧化，通过ＴＡＢ比色法测定丙二醛（ＭＤＡ）含量观测了４ＴＥＭＰＯ抗脂质过氧化作用。结果：ＯＴＭＰＯ（４氧２，２，６，６四甲基哌啶氮氧自由基）、ＨＴＭＰＯ（４羟２，２，６，６四甲基哌啶氮氧自由基）、ＨＴＭＰＯＨ（４羟２，２，６，６四甲基羟哌啶）、ＯＴＭＰＯＨ（４氧２，２，６，６四甲基羟哌啶）有较强的剂量依赖性抑制ＭＤＡ产生的作用，且ＯＴＭＰＯＨ，ＨＴＭＰＯＨ较ＯＴＭＰＯ，ＨＴＭＰＯ作用强，而ＯＴＭＰ（４氧２，２，６，６四甲基哌啶）、ＨＴＭＰ（４羟２，２，６，６四甲基哌啶）无效。结论：４ＴＥＭＰＯ有较明显的对抗脂质过氧化作用，作为一种临床抗自由基引起的脂质过氧化损伤的药物研究及开发利用，可能有广泛的前景。
OBJECTIVE: To study the anti-lipid peroxidation effects of 6 tetramethylpiperidine nitroxides and derivatives (4TEMPO), and to provide a basis for their further study and clinical application as anti-free radical damage drugs. Methods: Lipid peroxidation of rat liver cell membrane, myocardial cell membrane and liver mitochondria was induced by Fe2 + VitC. The anti-lipid peroxidation of 4TEMPO was observed by TAB colorimetric assay of malondialdehyde (MDA). Results: OTMPO (4oxy2,2,6,6methylpiperidine nitroxide), HTMPO (4hydroxy2,2,6,6methylpiperidine nitroxide ), HTMPOH (4 hydroxy 2,2,6,6 tetramethylpiperidine), OTMPOH (4 oxygen 2,2,6,6 tetramethylpiperidine) have a stronger dose Dependent inhibition of the role of MDA production, and OTMPOH, HTMPOH than OTMPO, HTMPO strong role, and OTMP (4 oxygen 2,2,6,6 tetramethylpiperidine), HTMP (4 hydroxy 2,2 , 6,6 tetramethylpiperidine) is invalid. CONCLUSION: 4TEMPO has a more obvious anti-lipid peroxidation effect. As a kind of anti-lipid-induced drug against lipid peroxidation, its research and development and utilization may have a broad prospect.