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为了解白菜型和甘蓝型冬油菜抗寒基因的表达差异,以6个抗寒性不同的冬油菜为材料,利用实时荧光定量PCR(qRT-PCR)分析常温对照以及4℃、-4℃和-8℃低温处理5d后Bn COR25、Bn ICE1和Cu/Zn-SOD三个基因的表达差异。结果表明,常温时白菜型与甘蓝型冬油菜Bn COR25、Bn ICE1和Cu/Zn-SOD基因未被激活,处于同一水平;随着温度的降低,白菜型冬油菜Bn COR25、Bn ICE1和Cu/Zn-SOD的相对表达量均上升,且均在-4℃时到达峰值;而甘蓝型冬油菜Bn COR25在4℃时到达峰值,Cu/Zn-SOD在-4℃时到达峰值,Bn ICE1的相对表达量在低温胁迫过程中始终低于常温水平;-8℃时白菜型与甘蓝型冬油菜各基因表达量均下降。在整个温度降低过程中,始终表现为白菜型冬油菜Bn COR25、Bn ICE1和Cu/Zn-SOD的相对表达量显著高于甘蓝型冬油菜,抗寒性强的品种高于抗寒性弱的品种。说明,白菜型与甘蓝型冬油菜间抗寒性的较大差异与这3个基因的表达有关。
In order to understand the differences of cold-tolerant genes between Brassica campestris and Brassica napus, six cold-tolerant winter rapeseed (Brassica napus L.) cultivars were used as materials to detect the expression of cold tolerance genes at 4 ℃, -4 ℃ and Differences of Bn COR25, Bn ICE1 and Cu / Zn-SOD expression after treatment at -8 ℃ for 5 days. The results showed that Bn COR25, Bn ICE1 and Cu / Zn-SOD genes of Brassica napus L. and Brassica napus L. were not activated at the same level at room temperature. With the decrease of temperature, Bn COR25, Bn ICE1 and Cu / The relative expression of Zn-SOD reached the peak at -4 ℃. The peak of Bn COR25 reached the peak at 4 ℃ and the peak at -4 ℃ in Bn ICE1 The relative expression level was always lower than normal temperature during low temperature stress. The gene expression levels of Brassica campestris and Brassica napus decreased at -8 ℃. During the whole process of temperature decrease, the relative expression of Bn COR25, Bn ICE1 and Cu / Zn-SOD in B. rapa was significantly higher than that in B. napus. The varieties with higher cold resistance were higher than those with cold hardiness Variety. This shows that the greater difference in cold resistance between cabbage type and Brassica napus is related to the expression of these three genes.