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目的 探讨枯否氏细胞在大鼠非酒精性脂肪性肝炎（ｎｏｎ－ａｌｃｏｈｏｌｉｃ ｓｔｅａｔｏｈｅｐａｔｉｔｉｓ， ＮＡＳＨ）发病中的作用。方法 １９只雄性ＳＤ大鼠随机分为模型组（１０只）和正常组（９只），分别予高脂肪饮食和标准饮食饲养１２周。ＨＥ染色观察肝组织切片病理学改变，透射电镜和溶菌酶免疫组织化学染色观察枯否氏细胞的数量和形态。结果 模型组大鼠均出现肥胖、高脂血症伴肝细胞大泡性脂肪变、小叶内炎症细胞浸润和坏死。与正常组相比，模型组肝小叶内抽否氏细胞数显著增加，并呈活化状态；模型组枯否氏细胞变化与其肝病理学改变相一致。结论 高脂饮食大鼠肝脏枯否氏细胞增多，并可能与其脂肪性肝炎的发病有关。
Objective To investigate the role of Kupffer cells in the pathogenesis of non-alcoholic steatohepatitis (NASH) in rats. Methods Twenty-nine male Sprague-Dawley rats were randomly divided into model group (n = 10) and normal group (n = 9), which were fed with high-fat diet and standard diet for 12 weeks respectively. The pathological changes of liver sections were observed by HE staining. The number and morphology of Kupffer cells were observed by transmission electron microscopy and lysozyme immunohistochemical staining. Results All the rats in the model group were obese, with hyperlipidemia and hepatocyte bullosa steatosis, inflammatory cell infiltration and necrosis in the lobules. Compared with the normal group, the model group, the number of lobular lobulation nodules increased significantly, and was activated; model group Kupffer cells and changes in liver pathology consistent. Conclusion Hypoglossal cells increase in the liver of high fat diet rats and may be related to the pathogenesis of steatohepatitis.