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将人胚食管胃移植于裸鼠皮下,期望建立一个研究食管胃部疾病的实验模型,人胚食管全层和粘膜层移植皮下均可成活(16/16),且均呈囊状生长,通过14个月的观察,从光镜、电镜、免疫组化、组织化学染色等方面分析,人胚食管在裸鼠体内的发育基本上与正常发育类似,且保持正常食管的基本组织结构和功能。人胚胃体部、窦部全层及体部粘膜皮下移植均没有成活(0/10),但窦部粘膜全部成活(12/12),移植片状粘膜也呈囊状生长。通过14个月的连续观察,基本保持窦部粘膜的组织结构和功能,通过腺体和CEA的变化,说明胚胃发育趋向成熟。研究中还探索了体外培养后再行移植,发现食管粘膜和窦部粘膜均可成活,且组织结构和功能与直接移植没有明显区别,为实验提供了方便。此外,为了达到长期移植的目的,而不受裸鼠寿命的限制,进行了移植物裸鼠间再移植,也可成活,且保持结构功能不变。
In order to establish an experimental model of esophageal and gastric diseases, the full-thickness human esophageal mucosa of human embryos and submucosal grafts could survive (16/16), all of which showed cystic growth through 14 months of observation, from the light microscope, electron microscopy, immunohistochemistry, histochemical staining and other aspects of human embryo development in nude mice basically similar to the normal development and maintain the normal structure and function of the normal esophagus. Human embryonic body, sinus full-thickness and mucosal subcutaneous transplantation did not survive (0/10), but all of the mucosal survival (12/12), transplanted mucosal also cystic growth. After 14 months of continuous observation, the histological structure and function of sinus mucosa were basically maintained. The changes of glandular and CEA showed that the development of embryo and stomach tend to mature. The study also explored in vitro culture and then transplant and found that the esophageal mucosa and sinus mucosa can survive, and the structure and function of the tissue and direct transplantation no significant difference for the convenience of the experiment. In addition, in order to achieve the purpose of long-term transplantation, without being limited by the life of nude mice, transplantation between nude mice was performed, which can also survive and maintain the structural function unchanged.