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目的:研究亚砷酸(As2O3)及联合顺铂(DDP)作用于人鼻咽癌裸鼠移植瘤后对RASSF1A表达的影响。方法:建立人鼻咽癌CNE2细胞裸鼠移植瘤模型,随机分为NaCl组、As2O3组、DDP组和As2O3加DDP联合组,对标本分别采用RT-PCR法检测RASSF1AmRNA的表达;免疫组织化学SP法检测RASSF1A蛋白的表达;HRM法检测RASSF1A启动子区甲基化率。结果:各实验组均能抑制人鼻咽癌CNE2裸鼠移植瘤生长,并能明显上调抑癌基因RASSF1A的表达,各实验组RASSF1A的甲基化率明显低于NaCl组,且As2O3与DDP联合优于As2O3、DDP单一用药。结论:As2O3抑制人鼻咽癌CNE2裸鼠移植瘤的生长,促进RASSF1A的表达。As2O3可抑制鼻咽癌细胞恶性表型和逆转RASSF1A的甲基化状态。
AIM: To investigate the effect of arsenious acid (As2O3) and cisplatin (DDP) on the expression of RASSF1A in human nasopharyngeal carcinoma xenografted in nude mice. Methods: The human nasopharyngeal carcinoma xenografted model of CNE2 cells was established and randomly divided into three groups: NaCl group, As2O3 group, DDP group and As2O3 plus DDP group. The expression of RASSF1A mRNA was detected by RT-PCR. Immunohistochemistry SP Method to detect the expression of RASSF1A protein; HRM assay RASSF1A promoter methylation rate. Results: The experimental groups all inhibited the growth of human nasopharyngeal carcinoma CNE2 xenografts and significantly up-regulated the expression of the tumor suppressor gene RASSF1A. The methylation rate of RASSF1A in each experimental group was significantly lower than that in NaCl group, and As2O3 and DDP combined Better than As2O3, DDP single drug. Conclusion: As2O3 can inhibit the growth of human nasopharyngeal carcinoma CNE2 xenografts in nude mice and promote the expression of RASSF1A. As2O3 inhibits the malignant phenotype of nasopharyngeal carcinoma cells and reverses the methylation status of RASSF1A.