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目的 建立一种高效毛细管电泳检测尿液中排泄肝素的方法 ,并用于检测膀胱肿瘤患者尿中的肝素排泄量。方法 采用磷酸缓冲体系的反极性毛细管电泳法 ,电压为 - 2 0 k V,检测波长为 190 nm,并用其测定 2 1例膀胱肿瘤患者及 12例健康者尿中排泄的肝素含量。实验数据经方差分析 ,显著性水平α=0 .0 5。结果 肝素峰面积和其含量呈良好的线性关系 ,尿中肝素排泄量 12例健康者为 (0 .94± 0 .48) μg/ L,2 1例膀胱肿瘤者中 11例 Ta～T1 期患者为 (1.30± 0 .73) μg/ L,10例 T2 ～T4期患者为 (0 .5 4± 0 .45 )μg/ L ,T2 ～ T4期者比 Ta～ T1 期及健康者显著降低 (P<0 .0 5 )。结论 反极性毛细管电泳法是一种分离人尿中肝素高效、快速、简便的方法 ,对膀胱肿瘤患者尿中肝素的检测发现 ,其排泄量的降低与膀胱肿瘤的浸润转移有关。
Objective To establish a high performance capillary electrophoresis method for the detection of excretion of heparin in urine and to detect urinary heparin excretion in patients with bladder cancer. Methods Reverse osmotic capillary electrophoresis with a phosphate buffer system was used. The voltage was -20 kV and the detection wavelength was 190 nm. The urinary excretion of heparin in 21 bladder cancer patients and 12 healthy controls were measured. The experimental data by analysis of variance, the significance level α = 0. Results Heparin peak area and its content showed a good linear relationship, urinary excretion of heparin in 12 cases (0.94 ± 0.48) μg / L, 21 cases of bladder tumor in 11 patients with stage T1 ~ T1 (1.30 ± 0.73) μg / L in 10 patients with T2 ~ T4 was (0.54 ± 0.45) μg / L, and those in T2 ~ T4 were significantly lower than those in Ta ~ T1 and healthy controls P <0. 05). Conclusion Reverse polarity capillary electrophoresis is an efficient, rapid and simple method for separating heparin from human urine. The detection of urinary heparin in bladder cancer patients shows that the decrease of excretion is related to the infiltration and metastasis of bladder tumor.