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目的:建立一个准确、简单、快速的分析人血浆中游离氨茶碱浓度的方法,并应用于临床治疗药物监测。方法:0.5 ml血浆样品经过中空纤维离心超滤(HFCF-UF)处理,以甲醇-10 mmol·L-1乙酸铵缓冲溶液(15/85,V/V)为流动相,采用Diamonsil C18色谱柱分离,流速为1.0ml·min-1,检测波长为275 nm,进样量为20μl。结果:血浆中游离氨茶碱浓度在0.25~25μg·ml-1范围内线性关系良好,最低定量限0.1μg·ml-1,日内、日间RSD分别小于1.1%和1.3%。结论:该方法简单、准确、重现性好,特别适合基层开展临床游离药物浓度的监测。该方法成功的应用于临床游离氨茶碱浓度的监测,为临床游离氨茶碱治疗药物监测提供了一个可靠的分析平台和进一步阐述游离药物浓度与药物疗效或毒性之间的关系奠定了基础。
OBJECTIVE: To establish an accurate, simple and rapid method for the analysis of free aminophylline in human plasma and to apply it in the clinical treatment of drug monitoring. Methods: 0.5 ml plasma samples were subjected to hollow fiber ultrafiltration (HFCF-UF) with methanol-10 mmol·L-1 ammonium acetate buffer solution (15/85, V / V) as mobile phase and Diamonsil C18 column The separation was performed at a flow rate of 1.0 ml · min-1 with a detection wavelength of 275 nm and an injection volume of 20 μl. Results: The plasma concentration of free aminophylline showed good linearity in the range of 0.25-25 μg · ml-1 with the lowest limit of quantitation of 0.1 μg · ml-1. The intra-day and inter-day RSD were less than 1.1% and 1.3%, respectively. Conclusion: The method is simple, accurate and reproducible. It is especially suitable for the monitoring of clinical free drug concentration in grass roots. The method is successfully applied to the monitoring of clinical free aminophylline concentration, which provides a reliable analytical platform for the clinical monitoring of free theophylline drugs and lays the foundation for further elucidation of the relationship between free drug concentration and drug efficacy or toxicity.