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选用１１个不育系、１３个恢复系以不完全双列杂交模式设计，进行杂交水稻组合的蛋白质含量测定及配合力、遗传力分析。结果表明杂交水稻不育系、恢复系蛋白质含量的σｇｃａ和σｓｃａ均达极显著，且受加性和非加性的多基因控制，非加性基因效应大于加性基因效应，育种值较小，即亲本的高蛋白质含量难于通过杂交遗传下去。杂交组合的ＳＣＡ效应值绝大多数为负值、非加性基因效应小，难于显示杂种优势。参配的不育系中， ０９１Ａ、 Ⅱ－３２Ａ、 Ｋ１７Ａ、 汕 Ａ蛋白质含量 ＧＣＡ和 ＳＣＡ的效应值大，是杂交水稻蛋白质育种的理想不育系。密阳 ４６、桂９９、 １５３９蛋白质含量的ＧＣＡ效应值大，是杂交水稻蛋白质育种的理想恢复系。 ＣＤＲ２２、多系１号、６０７８、明恢６３、明恢７７、沪恢５７、７０４蛋白质含量的ＳＣＡ值较高，以其配组有可能产生高蛋白组合，也是杂交水稻蛋白质育种的较好恢复系。
Eleven sterile lines were selected and 13 restorer lines were designed by incomplete double crosses to determine the protein content and the combining ability and heritability of the hybrid rice combinations. The results showed that both the σgca and σsca of the protein content of the CMS lines and restorer lines of hybrid rice reached extremely significant, and were controlled by additive and non-additive polygenes. The non-additive gene effect was greater than additive gene effect, breeding value was small, That is, the high protein content of the parent is difficult to inherit by crossing. Most of the SCA effect values of hybrid combinations were negative, and the non-additive gene effect was small, so it was difficult to show heterosis. Among the CMS lines, 091A, Ⅱ-32A, K17A and ShanA showed high effect of GCA and SCA, which were the ideal CMS lines for hybrid rice. The GCA effect of Miyang 46, Gui 99, 1539 is high, which is an ideal restorer line for the hybrid rice protein breeding. The SCA value of the protein content of CDR22, multifamily 1, 6078, Minghui 63, Minghui 77, Shanghai Hui 57,704 was higher, and the high SCA value could be produced by its combination, which is also a better recovery of the hybrid rice protein breeding system.