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目的 研究 型内皮素 (ET- 1)对人类子宫肌瘤细胞生长的作用 .方法 取人子宫肌瘤细胞培养传代 ,加入 1.0μmol· L- 1 ET- 1培养 ,1～ 4d后观察细胞增殖情况 ,并分别应用 1 2 5 I- ET- 1放射免疫结合法和免疫组化法检测 ET- 1受体(ETA)和 型内皮素受体 (ETB)蛋白合成 .结果 ET- 1刺激 1～ 3d后细胞数明显增多 .ET- 1处理后细胞 3H- Td R参入量明显增加 ,其变化趋势同细胞计数 .在培养细胞内检测到ETA蛋白 ,合成量随 ET- 1的加入而增加 ,第 4日达最大值 .ET- 1刺激前后所有细胞中未检测到 ETB蛋白产物 .结论 ET- 1促进子宫肌瘤细胞的生长和 DNA合成 ;ETA介导了ET- 1促细胞生长和增殖的过程 .
Objective To study the effect of endothelin (ET-1) on the growth of human uterine leiomyoma cells.Methods Human Uterine fibroids were passaged and cultured in 1.0μmol · L-1 ET-1 cells for 1 ~ 4 days. , And the protein synthesis of ET-1 receptor (ETA) and endothelin receptor (ETB) were detected by radioimmunoassay and immunohistochemistry respectively.Results ET-1 stimulated 1 ~ 3d The amount of 3H-TdR increased significantly after ET-1 treatment, and the trend was the same as the cell count.ETA protein was detected in cultured cells, and the amount of ET-1 increased with the addition of ET-1, ET-1 protein was not detected in all cells before and after stimulation with ET-1.Conclusion ET-1 can promote the growth of uterine fibroids and DNA synthesis. ETA mediates the process of ET-1 promoting cell growth and proliferation.