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目的探讨MN基因型定型在恶性血液病异基因造血干细胞移植植活证据检测中简单可行的方法。回顾性分析了10例患者恶性血液病异基因造血干细胞移植的临床资料。方法分别采取受者和供者移植前及移植后14天及365天的血液标本,根据已知基因序列设计合成引物,采取PCR-SSP的方法检测方法的检测受者和供者移植后的基因型和基因亚型。结果 10例患者MN为5例,MM为3例、NN为2例或MGMG2例,MTMT1例,MGMT6例,MTN1例。与血清学结果完全吻合。显示两组样本的表型与基因型吻合很好,资料的说明性较好(P>0.05)。结论血型基因检测的证据表明在同种异体造血干细胞移植中血型基因型可以作为早期监测的指标、标本用量少、科学敏感性强。
Objective To investigate the simple and feasible method of MN genotyping in the detection of graft-versus-graft evidence of allogeneic hematopoietic stem cell transplantation in patients with hematologic malignancies. The clinical data of 10 patients with hematologic malignancies allogeneic hematopoietic stem cell transplantation were retrospectively analyzed. Methods The blood samples of recipients and donors before transplantation and 14 days and 365 days after transplantation were respectively collected. Based on the known gene sequences, primers were designed and synthesized, and PCR-SSP method was used to detect the gene after transplantation Type and genotype. Results The MN in 10 cases was 5 cases, MM was 3 cases, NN was 2 cases or MGMG 2 cases, MTMT 1 case, MGMT 6 cases and MTN 1 case. Serologically consistent with the results. The results showed that the phenotypic and genotypic agreement of the two groups of samples was good, and the data were well descriptive (P> 0.05). Conclusion The evidence of blood group gene testing indicates that the blood genotypes can be used as indicators for early monitoring in allogeneic hematopoietic stem cell transplantation. The samples are used in small quantities and scientifically sensitive.