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目的通过测量Rho连接激酶(Rhoassociatedkinase,ROK)蛋白的含量来研究其在模拟失重大鼠股动脉收缩中可能发挥的作用。方法采用14d尾部悬吊大鼠(-30°)模拟失重效应,Wistar大鼠被随机分成两组:悬吊组(TS14d)和对照组(CON),利用离体血管环灌流技术和WesternBlotting技术检测股动脉平滑肌中ROK蛋白的表达和肌球蛋白轻链(MLC)磷酸化水平的变化。结果与对照组相比,14d尾部悬吊使大鼠股动脉中ROCK-Ⅱ蛋白的表达显著增高,ROCK-Ⅰ的表达量差异没有显著性;加入苯肾上腺素(PE)后,对照组股动脉MLC磷酸化水平显著高于悬吊组。结论14d尾部悬吊后大鼠股动脉中RhoA-ROK通路的活性增强,这可能在一定程度上对平滑肌收缩力减弱起到了代偿作用。
Objective To investigate the possible role of Rhoassociated kinase (ROK) protein in the contraction of the femoral artery in simulated weightless rats. Methods Wistar rats were randomly divided into two groups: the suspension group (TS14d) and the control group (CON). The rats were subjected to 14 days of tail suspension (-30 °) to simulate the effects of weightlessness. The peripheral blood vessel perfusion technique and Western Blotting technique Changes of ROK protein and myosin light chain (MLC) phosphorylation in femoral artery smooth muscle. Results Compared with the control group, the expression of ROCK-Ⅱprotein in the femoral artery of rats was significantly increased after 14 days of tail suspension. There was no significant difference in the expression of ROCK-Ⅰ between the control and the control group. After addition of phenylephrine (PE) MLC phosphorylation levels were significantly higher than the suspension group. Conclusion The RhoA-ROK pathway in the femoral artery of rats after 14 days tail suspension increases, which may play a compensatory role to the extent that the contractility of smooth muscle relaxes to some extent.