根据胸膜肺炎放线杆菌各血清型之间外毒素 (Apx) ,外膜脂蛋白 (OmlA) ,转铁蛋白B(TbpB)的基因差异 ,分别对各血清型进行PCR扩增 ,得到不同的特异性片段 ,可区分开生物Ⅰ型 13个标准菌株血清型中的 8个血清型。临床检测结果与血清学分型一致 ,将此分型系统用于临床送检的 12 6份肺脏和 4 2份扁桃体的病原学检测 ,可直接检测出胸膜肺炎放线杆菌感染。此方法还可以将一些尚未定型的菌株进行归类 ,弥补了血清学方法的不足 ,为细菌的流行病学调查提供了可靠的技术手段。 According to the gene differences of the exotoxin (Apx), OmlA and TbpB between the different serotypes of Actinobacillus pleuropneumoniae, each serotype was amplified by PCR and got different specific Sex fragments, can be distinguished from the serotypes of serotypes of 13 standard strains of biotype I. Clinical test results and serological classification consistent with the typing system for clinical examination of 12 6 lungs and 42 tonsil pathogen detection can be directly detected Actinobacillus pleuropneumoniae infection. This method can also classify some unsettled strains to make up for the lack of serological methods and provide a reliable technical means for the epidemiological investigation of bacteria.