1 Source The sequence was determined from a reverse PCR product, which was ligated to pMD18-T vector(TaKaRa Biotechnology Co.), from nuclear genomic DNA of “Chinese Spring” wheat (Triticum aestivum).rn2 Name and description Prolamins, which are important storage proteins in cereal grains, are synthesized in the endosperm under strictly tissue-specific and temporal transcription control. Several putative regulatory elements or motifs in their promoters have been identified and one of them is a 7 bp element (5′TGTAAAG 3′) termed the Prolamin Box (P-box). The protein, named prolamin-box binding factor or PBF that binds to the P-box, has also been found. BPF contains the highly conserved Cys2-Cys2 zinc finger motif characteristic of the discovered DOF (DNA binding with One Finger) class of the DNA-binding proteins. Recently the cloning of a wheat endosperm-specific cDNA, encoding PBF, has been reported. The nucleotide sequence (2 461 bp) of promoter and partial CDS(49 bp) of wheat PBF is showed in this paper. CAAT box is at -82 to-78 and TATA box at -949 to -944. The amino acid sequence deduced from partial CDS is “MEEVFPSNSKSKAGQM”.rn