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目的:探讨背侧纹状体(dorsal striatum)内促肾上腺皮质激素释放因子(CRF)阳性轴突终末和CRF表达的分布特征及年龄变化。方法:采用免疫组织化学、原位杂交、免疫印迹和体视学分析方法,观察分析出生后第1~21日龄(P1-21)及成年(P90)大鼠背侧纹状体内的CRF轴突终末及CRF蛋白含量。结果:背内、背外侧纹状体内含大量CRF轴突终末,背外侧纹状体内的CRF轴突终末更为丰富。这些终末集中位于纹状体的尾部区域,与纹状体主要神经元形成典型的环胞体支配。和成年比较,发育早期(P1-14)背侧纹状体内CRF轴突终末的密度较高(P<0.05),CRF蛋白表达量以P7组相对最高(P<0.05)。结论:发育早期背侧纹状体内富含CRF轴突终末,提示CRF是影响纹状体神经元生长发育的因子之一。
Objective: To investigate the distribution and age distribution of corticotropin-releasing factor (CRF) -positive axon terminals and CRF in dorsal striatum. Methods: Immunohistochemistry, in situ hybridization, Western blotting and stereological methods were used to observe the CRF axis in the dorsal striatum of the first to 21st days (P1-21) and the adult (P90) Sudden terminal and CRF protein content. Results: A large number of terminals of CRF axons were present in the dorsal and medial dorsal striatum. The terminals of CRF axons in the dorsal lateral striatum were more abundant. These terminals are centrally located in the caudal region of the striatum and form typical circadian bodies within the major neurons of the striatum. Compared with the adult, the density of CRF axons in the dorsal striatum at the early stage of development (P1-14) was higher (P <0.05) and the expression of CRF was highest in the P7 group (P <0.05). Conclusion: Early dorsal striatum is rich in terminal CRF axons, suggesting that CRF is one of the factors that affect the growth and development of striatal neurons.