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微孔比色法采用合成的磷脂类似物２－硫代十六酰乙基磷酸胆碱作底物，在多孔聚苯乙烯板的小孔中反应，并用酶联免疫检测器连续测定和记录吸收值．同时应用此法及滴定法检测酶活力，从猪胰腺中制备了一种分子量低（１４．３ｋＤ），对热、酸稳定，活性依赖Ｃａ２＋的ＰＬＡ２．两种方法检测结果具有可比性，而微孔比色法同时可测多个样品，有节约样品，灵敏度较高等优点．微孔比色法特别适用于大量的样品测定，如拮抗剂筛选、临床样品及制备酶时层析级分的检测等．
Micropore colorimetric method Using synthetic phospholipid analog 2-thiohexadecanoyl ethyl phosphorylcholine as a substrate, react in the wells of a porous polystyrene plate and measure continuously with an enzyme-linked immunosorbent and record the absorbance value. At the same time, this method and titration method were used to detect the activity of PLA2. A low molecular weight (14.3kD) PLA2 was prepared from porcine pancreas and was stable to heat, acid and activity. Two methods test results are comparable, and microporous colorimetric simultaneous measurement of multiple samples, save the sample, the advantages of higher sensitivity. Microporous colorimetric method is particularly suitable for a large number of sample determination, such as antagonist screening, clinical samples and the preparation of enzyme chromatography fraction detection.