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目的 :探讨CD3单抗 (mAb)和淋巴细胞功能相关抗原 1单抗 (LFA 1mAb)对狼疮肾炎 (LN)患者外周血单个核细胞 (PBMC)的共刺激作用。 方法 :2 9例LN患者被分为活动期 (n =14)和非活动期 (n =15 ) ,以 12例健康献血员为对照 ,观察CD3mAb或 (和 )LFA 1mAb共刺激及阻断 1 磷酯酰肌醇 3 激酶 (PI3 K)对体外培养 96h后PBMC增生和IL 2产生的影响。PBMC提取采用Ficoll密度梯度离心法 ,细胞增生实验采用3H TdR掺入法 ,IL 2测定采用ELISA法。 结果 :PBMC培养 96h后 ,自然生长的LN非活动期和活动期PBMC3H TdR掺入量和IL 2合成与正常对照无明显差异 (P值均 >0或 0 0 5 ) ;与自然生长的PBMC相比 ,CD3mAb单独处理增加了LN非活动期 (P值均 <0 0 5 )和活动期 (P值均 <0 0 5 )PBMC3H TdR掺入量和IL 2合成。而单独CD3mAb对正常对照PBMC3H TdR掺入量和IL 2合成没有影响 (P值均 >0 0 5 )。与CD3mAb单独处理组相比 ,CD3mAb和LFA 1mAb共刺激均增加了LN活动期 (P值均 <0 0 5 )和非活动期 (P值均 <0 0 5 )及正常对照 (P值均 <0 0 5 )PBMC3H TdR掺入量和IL 2合成 ;与对照组相比 ,CD3mAb和LFA 1mAb共刺激后活动期 (P值均 <0 0 1)和非活动期 (P值均 <0 0 1)PBMC3H TdR掺入量和IL 2合成均增加 ,但活动期效应明显强于非活?
Objective: To investigate the costimulatory effect of CD3 monoclonal antibody and LFA 1 mAb on peripheral blood mononuclear cells (PBMCs) in patients with lupus nephritis (LN). Methods: 29 LN patients were divided into active phase (n = 14) and inactive phase (n = 15). 12 healthy blood donors were used as control group to observe the co-stimulation of CD3 mAb or (and) LFA 1 mAb and block 1 Effect of phosphatidylinositol 3 - kinase (PI3K) on proliferation of PBMCs and production of IL - 2 after 96 hours in vitro. PBMCs were extracted by Ficoll density gradient centrifugation, 3H TdR incorporation was used for cell proliferation assay, and ELISA was used for determination of IL-2. Results: After PBMCs were cultured for 96h, there was no significant difference in the activities of naturally occurring LN and inactive PBMCs among the three groups (P> 0 or 0 05) Compared with the control group, CD3 mAb alone increased the incorporation of PBMC3H TdR and IL 2 synthesis in inactive LN (P <0.05) and active phase (P <0.05). However, CD3mAb alone had no effect on the incorporation of PBMC3H TdR and IL2 synthesis (P> 0.05). Both CD3 mAb and LFA 1 mAb co-stimulated LN activity (both P <0.05) and inactive (P <0.05) and normal controls (P <0.05), compared with CD3 mAb alone Compared with the control group, CD3mAb and LFA 1mAb co-stimulated active phase (P <0.01) and inactive phase (P <0 0 1) ) PBMC3H TdR incorporation and IL 2 synthesis increased, but the activity of the period was significantly stronger than inactivated?