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应用非同位素斑点杂交及真彩色医学图像分析系统的定量分析等技术，观察了肾上腺皮质脂类含量及肾上腺组织中甾体激素合成过程中的关键酶－３β－羟脱氢酶Δ５－４异构酶（３β－ＨＳＤ）ｍＲＮＡ的表达，以期探讨甘草次酸拮抗外源性糖皮质激素对肾上腺皮质反馈抑制作用的机理。ＳＤ大鼠及阿霉素注射制成的肾病鼠接受甘草次酸和（或）地塞米松前后测定动物血浆皮质酮含量、肾上腺皮质束状带脂类含量及肾上腺３β－ＨＳＤｍＲ－ＮＡ的表达。结果发现地塞米松可导致以上三项指标下降，而甘草次酸能促进３β－ＨＳＤｍＲＮＡ的表达和增加脂类含量，且二者呈正相关（ｒ＝０．８４３１，Ｐ＜０．０５），脂类含量又与血浆皮质酮水平正相关（ｒ＝０．６８８２，Ｐ＜０．０１）。提示甘草次酸可促进肾上腺组织中３β－ＨＳＤｍＲＮＡ表达及增加脂类含量，使肾上腺皮质激紊的合成和分泌增加，从而拮抗外源性皮质激素所致的肾上腺皮质反馈抑制现象。
Using non-isotopic dot blot hybridization and true color medical image analysis system quantitative analysis and other techniques to observe the adrenocortical lipid content and adrenal steroid hormone synthesis process key enzyme -3β-hydroxy desaturase Δ5-4 isomerization (3β-HSD) mRNA expression in order to explore glycyrrhetinic acid antagonism of exogenous glucocorticoid on adrenal cortex feedback inhibition mechanism. SD rats and doxorubicin injection nephropathy rats received glycyrrhetinic acid and (or) dexamethasone before and after the determination of animal plasma corticosterone content, adrenal cords with lipid content and adrenal 3β-HSDmR-NA expression. The results showed that dexamethasone can lead to the decline of the above three indicators, and glycyrrhetinic acid can promote 3β-HSD mRNA expression and increase lipid content, and both were positively correlated (r = 0.8431, P <0.05), lipid The level of plasma corticosterone was positively correlated with plasma corticosterone level (r = 0.6882, P <0.01). Prompted glycyrrhetinic acid can promote adrenal 3β-HSDmRNA expression and increase lipid content, the synthesis and secretion of adrenal cortex turbulence increased, thus antagonizing the exogenous cortical hormone-induced adrenal cortical feedback inhibition.