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为了获得适合流式细胞仪检测的样品。通过碘化丙啶标记和流式细胞仪对食管癌细胞内DNA含量分布进行分析 ,在不同的时间内对血液中淋巴细胞内DNA含量的测定结果进行研究。结果显示 :结构完整的细胞DNA的荧光点图形成二倍体、四倍体的区域。组A各样品具有良好一致性 ,1～ 3天内差异小 ;组B各样品差异较大。因此细胞的完整性是DNA含量检测的基本条件。细胞经PI染色后若不能及时检测 ,可避光、4℃放置 1～ 3天仍可获得较理想的实验结果。
In order to obtain a suitable sample for flow cytometry. The distribution of DNA in esophageal cancer cells was analyzed by propidium iodide labeling and flow cytometry, and the results of determination of DNA content in the blood lymphocytes were studied at different times. The results showed that: the structural integrity of the cellular DNA fluorescence dot map formed diploid, tetraploid region. Group A samples have good consistency, small differences within 1 ~ 3 days; group B samples vary greatly. Therefore, the integrity of the cell DNA content of the basic conditions for testing. If cells can not be detected in time by PI staining, they can be protected from light and the ideal experimental results can still be obtained after being placed at 4 ° C. for 1 to 3 days.