论文部分内容阅读
目的:探讨IL-2对CD4+CD25+调节性T细胞(Tregs)的增殖及功能的影响。方法:提取B6小鼠脾脏细胞,流式细胞仪分离CD4+CD25+Tregs,将新鲜分离的CD4+CD25+Tregs与抗CD3单克隆抗体、同种同系抗原递呈细胞(APCs)及外源性IL-2共同培养,测定其增殖活性;并检测体外扩增后的CD4+CD25+Tregs的免疫抑制活性及其Foxp3的表达。结果:与外源性IL-2共同培养的CD4+CD25+Tregs增殖程度强烈,与对照组比较,差异有统计学意义(P<0.05);体外扩增的CD4+CD25+Tregs抑制CD4+CD25-T细胞增殖活性的能力与新鲜分离的CD4+CD25+Tregs相似(P>0.05)。体外扩增的CD4+CD25+Tregs的Foxp3表达与新鲜分离的CD4+CD25+Tregs亦相似(P>0.05)。结论:外源性IL-2能够消除CD4+CD25+Tregs的无反应状态,且体外扩增的CD4+CD25+Tregs保持了其抑制活性。
Objective: To investigate the effect of IL-2 on the proliferation and function of CD4 + CD25 + regulatory T cells (Tregs). Methods: The spleen cells of B6 mice were isolated and CD4 + CD25 + Tregs were isolated by flow cytometry. Freshly isolated CD4 + CD25 + Tregs and anti-CD3 monoclonal antibodies, allogeneic antigen presenting cells (APCs) IL-2 were co-cultured to measure their proliferative activity. The immunosuppressive activity and Foxp3 expression of CD4 + CD25 + Tregs in vitro were detected. Results: The proliferation of CD4 + CD25 + Tregs co-cultured with exogenous IL-2 was strong, and the difference was statistically significant (P <0.05); CD4 + CD25 + Tregs expanded in vitro The ability of T cells to proliferate was similar to freshly isolated CD4 + CD25 + Tregs (P> 0.05). Foxp3 expression of CD4 + CD25 + Tregs expanded in vitro was also similar to freshly isolated CD4 + CD25 + Tregs (P> 0.05). Conclusion: Exogenous IL-2 can abolish the non-responsive state of CD4 + CD25 + Tregs, and the in vitro expanded CD4 + CD25 + Tregs maintain their inhibitory activity.