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目的探讨纤支镜肺泡灌洗液中结核分枝杆菌DNA在涂阴肺结核诊断中的价值。方法应用荧光定量聚合酶链反应(FQ-PCR)技术对300例涂阴肺结核,178例菌阳肺结核及119例肺炎或肺癌患者的纤支镜肺泡灌洗液标本进行结核分枝杆菌DNA检测。结果3种病因肺泡灌洗液结核分枝杆菌DNA阳性检出率分别为:74.3%(223/300)、94.9%(169/178)、17.6%(21/119)。结论荧光定量聚合酶链反应检测肺泡灌洗液结核分枝杆菌DNA,用于肺结核诊断可提高肺结核诊断的敏感性和准确性,明显优于痰抗酸染色,亦较痰结核菌培养诊断灵敏、快速,可作为肺结核诊断较可靠指标。
Objective To investigate the value of Mycobacterium tuberculosis DNA in bronchoalveolar lavage fluid in the diagnosis of smear-negative pulmonary tuberculosis. Methods FQ-PCR was used to detect Mycobacterium tuberculosis DNA in 300 cases of smear-negative pulmonary tuberculosis, 178 cases of positive pulmonary tuberculosis and 119 cases of bronchoscopy bronchoalveolar lavage fluid in patients with pneumonia or lung cancer. Results The positive rates of DNA of M. tuberculosis in the three causes of bronchoalveolar lavage were 74.3% (223/300), 94.9% (169/178) and 17.6% (21/119), respectively. Conclusion Fluorescent quantitative polymerase chain reaction for the detection of M. tuberculosis DNA in pulmonary lavage fluid for the diagnosis of pulmonary tuberculosis can improve the sensitivity and accuracy of diagnosis of pulmonary tuberculosis and is significantly better than sputum acid- Fast, can be used as a more reliable indicator of pulmonary tuberculosis diagnosis.