To survey the tea resource advantages in Guizhou’s mountainous areas and complete rational layout of pollution-free tea planting areas, we take the case of Guiding County in Guizhou Province to establish the comprehensive evaluation indicator system of pollution-free land for tea plantation and comprehensive evaluation model of soil suitability of land for tea plantation according to the Environmental Conditions Standard for Pollution-free Food-Tea Producing Areas by the Ministry of Agriculture (NY 5020-2001), using land use data, remote sensing data, soil data, temperature, precipitation, light, DEM and other critical basic data, combined with the spatial analysis statistical method. The results show that the area of land suitable for planting tea is 1265 km2, accounting for 77.57% of the entire study area; the area of land with soil quality, climatic conditions and terrain conditions to meet the planting standard of pollution-free tea is 1232.51 km2, accounting for 75.57% of the total land area; the area of land most suitable for the planting of pollution-free tea is 128.71 km2, accounting for 7.89% of the total land area. The above studies can provide scientific basis and decision support for the implementation of tea planting industry standards in the specific areas, and provide reference for the comprehensive assessment of tea planting in other areas. To survey the tea resource advantages in Guizhou’s mountainous areas and complete rational layout of pollution-free tea planting areas, we take the case of Guiding County in Guizhou Province to establish the comprehensive evaluation indicator system of pollution-free land for tea plantation and comprehensive evaluation model of soil suitability of land for tea plantation according to the Environmental Conditions Standard for Pollution-free Food-Tea Producing Areas by the Ministry of Agriculture (NY 5020-2001), using land use data, remote sensing data, soil data, temperature, precipitation, light, DEM and other critical basic data, combined with the spatial analysis statistical method. The results show that the area of ​​land suitable for planting tea is 1265 km2, accounting for 77.57% of the entire study area; the area of ​​land with land soil quality, climatic conditions and terrain conditions to meet the planting standard of pollution-free tea is 1232.51 km2, accounting for 75.57% of the tota The area of ​​land most suitable for the planting of pollution-free tea is 128.71 km2, accounting for 7.89% of the total land area. The above studies can provide scientific basis and decision support for the implementation of tea planting industry standards in the specific areas, and provide reference for the comprehensive assessment of tea planting in other areas.