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目的探讨硝酸甘油(GTN)作为一氧化氮供体对人宫颈癌Hela细胞增殖及全细胞蛋白质组学表达的调控意义。方法分别用细胞形态学和噻唑蓝比色法(MTT)观察经GTN作用的Hela细胞生长和增殖状况;双向凝胶电泳技术分离Hela细胞蛋白质,观察GTN对细胞蛋白表达谱的影响。结果培养液中GTN浓度为40μg/ml时Hela细胞分离、皱缩,出现小球状或异型性,细胞外杂质增多,密度明显下降(0.0825,P<0.05);MTT检测细胞增殖抑制率为73.3%;双向电泳分析显示GTN组与对照组Hela细胞蛋白质点匹配率为86%,GTN组较对照组312个(47%)蛋白点表达明显改变,101个(15.2%)上调,211个(31.8%)下调,且有17个蛋白点只在GTN组细胞中表达,21个蛋白点只在对照组细胞中表达。结论一定浓度的GTN能够抑制肿瘤细胞的生长和调控细胞内蛋白质表达。
Objective To investigate the regulatory effect of nitroglycerin (GTN) as a nitric oxide donor on human cervical cancer Hela cell proliferation and whole cell proteomics. Methods The growth and proliferation of Hela cells treated with GTN were observed by MTT assay. The proteins of Hela cells were separated by two-dimensional gel electrophoresis and the effect of GTN on the protein expression profile was observed. Results Hela cells isolated and shrunken with a concentration of 40μg / ml of GTN in culture medium showed small globular or atypia, increased extracellular extracellular matrix density and decreased density (0.0825, P <0.05). The inhibition rate of MTT assay was 73.3% Two-dimensional electrophoresis analysis showed that the protein spots of Hela cells in GTN group and control group were 86%, 312 (47%) in GTN group were significantly increased, 101 (15.2%) were up-regulated, 211 ), And 17 protein spots were only expressed in GTN group cells, while 21 protein spots were only expressed in control group cells. Conclusion A certain concentration of GTN can inhibit tumor cell growth and regulate intracellular protein expression.