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采用氢气清除法测定胃粘膜血流量（ｇａｓｔｒｉｃｍｕｃｏｓａｌｂｌｏｏｄｆｌｏｗ，ＧＭＢＦ），观察大鼠胃内灌注低浓度盐酸酒精（０．１５ｍｏｌ／ＬＨＣｌ＋１５％ＥｔＯＨ）后灌注高浓度盐酸酒精（０．６ｍｏｌ／ＬＨＣｌ＋１５％Ｅｔｏｌｌ）对ＧＭＢＦ和胃粘膜损害的影响，以及ＧＭＢＦ在适应性细胞保护中的作用。结果如下：（１）先用低浓度盐酸酒精作为弱刺激灌注随之以高浓度盐酸酒精作为强刺激灌注，引起胃粘膜适应性保护现象，它表现为：大体损伤和损伤深度分别比单独强刺激组减少４７．０９％和４４．５７％（Ｐ＜０．０１，Ｐ＜０．０５）；与此同时，ＧＭＢＦ亦出现适应性增强反应，即比单独强刺激组增加２８．０２％（Ｐ＜０．０５）；（２）近胃动脉灌注血管加压素后，ＧＭＢＦ的适应性增强反应被阻断，对胃粘膜的适应性保护作用也消失；（３）静脉注射消炎痛后，ＧＭＢＦ的适应性增强反应被阻断，对胃粘膜的适应性细胞保护作用消失，粘膜损伤程度加重。上述结果表明：低浓度盐酸酒精作为弱刺激引起的适应性细胞保护作用与ＧＭＢＦ增加反应增强有关；减弱或阻断这一增强效应可使适应性细胞保护作用消失；内源性前列腺素参与适应性细胞保护作用可能部分与ＧＭＢＦ增加有关。
Gastric mucosal blood flow (GMBF) was determined by hydrogen scavenging method. After the gastric perfusion with low concentration of hydrochloric acid alcohol (0.15mol / LHCl + 15% EtOH) and high concentration of hydrochloric acid (0.6mol / LHCl + 15% EtOH) GMBF and gastric mucosal damage, and the role of GMBF in adaptive cytoprotection. The results are as follows: (1) Low concentration of hydrochloric acid alcohol as a weak stimulus perfusion followed by high concentration of hydrochloric acid alcohol as a strong stimulus perfusion, causing gastric mucosal adaptive protection phenomenon, which showed: general injury and injury depth separately than strong stimulation Group decreased by 47.09% and 44.57%, respectively (P <0.01, P <0.05). At the same time, GMBF also showed adaptive enhancement reaction, which increased by 28.02% <0.05). (2) After the proximal gastric artery was infused with vasopressin, the adaptive enhancement of GMBF was blocked and the protective effect on gastric mucosa disappeared. (3) After intravenous injection of indomethacin, GMBF The adaptive enhancement reaction was blocked, the adaptive protective effect of gastric mucosa disappeared, mucosal damage increased. The above results indicate that the adaptive cytoprotective effect of low concentration of hydrochloric acid as a weak stimulus may be related to the increased response of GMBF; the protective effect of adaptive cytoprotection may be abolished by the weakening or blocking of this enhancing effect; the endogenous prostaglandin is involved in the adaptability Cytoprotection may be partly related to the increase of GMBF.