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采用Biolog方法研究了砷(As)矿区农田土壤微生物碳源利用多样性及其与土壤化学性质的关系.结果表明:3种土壤的N、P、K、有机质(OM)、Cu和Zn全量依次为中As>高As>低As土壤.中As和高As土壤微生物平均吸光度以及多样性指数(H’、D、U)显著高于低As土壤.主成分分析与生理碳代谢指纹图谱分析表明,中As和高As土壤微生物对糖类、氨基酸类等碳源的利用程度显著高于低As土壤.典范对应分析显示,影响土壤微生物群落碳源代谢的主要有全P、OM、全Pb、全Zn、全N和pH,全As并不是最主要的影响因子.可见,养分是影响长期污染土壤微生物群落结构和功能多样性的主要因素.
Biolog was used to study the diversity of soil microbial carbon sources and its relationship with soil chemistry in arsenic (As) mining area. The results showed that the contents of N, P, K, OM, Cu, (H ’, D, U) in As and As soil were significantly higher than those in low-As soil. The analysis of principal component analysis and physiological carbon metabolism fingerprinting showed that the average absorbance and diversity index , As and As soil microorganisms have higher utilization of carbohydrates, amino acids and other carbon sources than low As soil.Analysis of canonical correspondence shows that the main source of carbon metabolism in soil microbial communities are all P, OM, total Pb, Total Zn, total N, pH, and total As are not the most important influencing factors.It can be seen that nutrient is the main factor that affects the structure and functional diversity of long-term contaminated soil microbial community.