论文部分内容阅读
研制了一种用于促黄体生成素(LH)检测的电化学免疫传感器,实现了促黄体生成素的高灵敏度和快速检测需求。采用纸基免疫传感器作为基底材料,氨基化石墨烯(NH2-G)/硫堇/纳米金复合物修饰的丝网印刷工作电极(SPWE)作为检测系统的工作电极。该免疫传感器的检测原理是:电化学物质硫堇通过电极表面氧化还原反应产生电化学电流,抗原抗体的特异性结合会影响电化学电流的释放,导致电流下降,并且检测电流与抗原的浓度呈反比。实验结果表明:对于促黄体生成素在1~100 mIU·mL~(-1)内该传感器具有很好的检测结果,检测的最低限为1 mIU·mL~(-1),检测的线性系数为0.991,并且检测时间可以控制在10 min。因此该传感器可用于促黄体生成素的快速和高灵敏度检测需求,这对于性激素水平的动态监测,进而实现不孕不育疾病个性化诊断治疗以及指导备孕都有着重要的意义。
An electrochemical immunosensor for the detection of luteinizing hormone (LH) was developed to achieve the high sensitivity and rapid detection of luteinizing hormone. A paper-based immunosensor was used as the substrate material. Amino-graphene (NH2-G) / thionine / nano-gold complex modified screen printed working electrode (SPWE) was used as the working electrode of the detection system. The detection principle of the immunosensor is that the electrochemical substance thionine generates electrochemical current through redox reaction on the surface of the electrode, and the specific binding of the antigen antibody will affect the release of the electrochemical current, resulting in the decrease of the current, and the detection current and the concentration of the antigen Inverse. The experimental results show that the sensor has good detection results for luteinizing hormone in the range of 1 ~ 100 mIU · mL -1, the detection limit is 1 mIU · mL -1, the linear coefficient of detection Is 0.991, and the detection time can be controlled at 10 min. Therefore, the sensor can be used for rapid and high sensitivity detection of luteinizing hormone, which is of great significance for the dynamic monitoring of sex hormone levels, and then for the personalized diagnosis and treatment of infertility diseases and for the guidance of pregnancy preparation.