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本文研究了新试剂安替比林基重氮氨基-2,4-二硝基苯(APDNBT)与汞的显色反应.在Tween-80存在下和pH=10.2～12.3的缓冲溶液中,APDNBT与汞生成黄色络合物,其络合比为Hg:APDNBT=1:2.建立了以445nm为参比波长,538nm为测量波长的双波长分光光度法,其表观摩尔吸光系数为2.1×10~5 L·mol~(-1)·cm~(-1),线性范围是0～15μg/25mL.成功地测定了天然水样及尿液中的汞,标准加入回收率为97%～104%,结果的相对标准偏差小于2.3%.本文研究了新试剂安替比林基重氮氨基-2,4-二硝基苯(APDNBT)与汞的显色反应.在Tween-80存在下和pH=10.2～12.3的缓冲溶液中,APDNBT与汞生成黄色络合物,其络合比为Hg:APDNBT=1:2.建立了以445nm为参比波长,538nm为测量波长的双波长分光光度法,其表观摩尔吸光系数为2.1×10~5L·mol~(-1)·cm~(-1),线性范围是0～15μg/25 mL.成功地测定了天然水样及尿液中的汞,标准加入回收率为97%～104%,结果的相对标准偏差小于2.3%.
In this paper, the color reaction of the new reagent antipyrine amino-2,4-dinitrobenzene (APDNBT) with mercury was studied.Under the condition of Tween-80 and the buffer solution of pH = 10.2 ~ 12.3, APDNBT The complex formed with mercury was Hg: APDNBT = 1: 2. The dual-wavelength spectrophotometry with 445nm as the reference wavelength and 538nm as the measuring wavelength was established and the apparent molar absorptivity was 2.1 × 10 ~ 5 L · mol -1 · cm -1 .The linear range was 0 ~ 15μg / 25mL.The mercury in natural water samples and urine samples was successfully measured and the standard addition recovery was 97% 104%, and the relative standard deviation (RSD) of the results was less than 2.3% .In this paper, the color reaction of the new reagent diacetylamino-2,4-dinitrobenzene (APDNBT) with mercury was studied in the presence of Tween-80 And pH = 10.2 ~ 12.3, APDNBT formed a yellow complex with mercury with a complex ratio of Hg: APDNBT = 1: 2. The dual-wavelength spectrometer with 445nm as the reference wavelength and 538nm as the measuring wavelength The apparent molar absorptivity was 2.1 × 10 ~ 5 L · mol -1 (-1) · cm -1 and the linear range was 0 ~ 15 μg / 25 mL.It was successfully determined that natural water and urine In the mercury, the standard recovery rate of 97% to 104%, the results of the relative Standard deviation is less than 2.3%.