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在Grocott改良的果氏四胺银方法中,分别以5％过碘酸钠与5％铬酸作氧化剂,对感染卡氏肺孢子虫的小鼠肺涂片、大鼠及病人的肺切片进行染色。小鼠肺涂片的氧化时间分别为5,15与60min,温度为20℃;银浸时间90min,温度60℃。在较短氧化时间即15min以下时,卡氏肺孢子虫包囊显示淡褐色或深褐色,部分包囊内清晰可见一对括号状结构;但氧化时间较长时,包囊呈黑色且显示受损。在同一批次小鼠肺涂片氧化时间为5min时,过碘酸钠氧化较之铬酸显示较多的含括号状结构的包囊。在过碘酸钠氧化的大鼠或病人的肺切片中,也可见到该结构。实验结果表明以过碘酸钠作氧化剂进行银染反应优于铬酸,且可省略亚硫酸氢钠处理步骤,这将有利于临床应用。
In the Grocott modified fructo-tetramisodium method, lung smears infected with Pneumocystis carinii, lung sections of rats and patients were treated with 5% sodium periodate and 5% chromic acid as oxidants, respectively dyeing. Mouse lung smear oxidation time were 5,15 and 60min, the temperature was 20 ℃; silver immersion time 90min, temperature 60 ℃. Pneumocystis carinii cysts showed a light brown or dark brown color at short oxidative time, ie 15 min, and a pair of bracket-like structures were clearly visible in some of the cysts. However, when the oxidation time was longer, the cysts were black and showed damage. In the same batch of mice lung smear oxidation time of 5min, sodium periodate oxidation than chromic acid showed more brackets containing the structure of the cyst. This structure is also seen in lung sections of sodium or iodide-oxidized rats or patients. The experimental results show that silver stain with sodium periodate as oxidant is better than chromic acid, and the step of sodium bisulfite treatment can be omitted, which will be helpful for clinical application.