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1987年6月,我们用50例病区鼠,20例非病区鼠做了光镜病理形态学及IgG免疫酶标的观察。实验组:HE染色发现50例家鼠心肌不同程度地间质水肿、单核细胞、淋巴细胞浸润、颗粒样变性,其中9例伴有小灶性的凝固性坏死和肌溶解及片状瘢痕灶。冰冻切片免疫酶染色标本50例中的12例发现有酶颗粒,呈棕褐色,颗粒大小不一致,均存在于心肌细胞的胞浆内,在12例IgG阳性标本中,9例是在石蜡切片HE染色标本中所见的肌溶解和瘢痕形成,其余3例在HE切片中只表现各种变性、水肿及细胞浸润。对照组:HE切片中仅3例见颗粒样变性,IgG切片中仅见1例阳性,其余都正常。通过克山病区50例家鼠心肌免疫酶标的观察,发现有24％的标本存在IgG沉积,证明了病区家鼠心肌与病人心肌存在同样的IgG沉积及同样的心肌病理性改变。这一现象说明人与家鼠在同一地区条件下不同程度地都受到某些因素损害,以致产生同一结果。许多调查表明,克山病区常伴有动物缺硒病,此外,用克山病区粮喂养的动物其心肌坏死的检出率较非病区明显增加。实验表明病区鼠心肌损害和IgG沉积明显高于对照组,这与病区病人心肌损害及病人心肌IgG沉积是一致的,因此,我们认为心肌损伤与病区硒、粮、水因素有密切关系。心肌损伤后,导致继发性免疫病理过程产生。
In June 1987, we used 50 ward rats and 20 non-ward rats to observe the histopathology and IgG immunoenzyme. In the experimental group, HE staining showed that interstitial edema, mononuclear cells, lymphocyte infiltration and granular degeneration were found in 50 cases of myocardium in house mouse, of which 9 cases were associated with focal coagulation necrosis and muscle lysis and flake scar focus. Frozen section immunostained specimens of 50 cases of 12 cases found enzyme particles, was brown, particle size inconsistencies, are present in the cytoplasm of myocardial cells, 12 cases of IgG-positive specimens, 9 cases were paraffin sections HE Myostomy and scarring were seen in the stained specimens, and the remaining three showed only a variety of degeneration, edema and cell infiltration in HE sections. In the control group, only 3 of the HE sections showed granulocyte-like degeneration, only 1 of the IgG sections was positive and the rest were normal. Myocardial immunoblotting was performed in 50 cases of domestic mice in Keshan disease area. It was found that 24% of the samples had IgG deposition, indicating that there was the same IgG deposition and the same pathological changes of myocardium in the myocardium of rats and rats. This phenomenon shows that humans and rats and rats are affected to some extent under the same regional conditions by some factors, resulting in the same result. Many surveys show that the Keshan ward is often accompanied by animals lacking selenium disease. In addition, the detection rate of myocardial necrosis in animals fed with food in Keshan ward was significantly higher than that in the non-ward. Experiments show that ward myocardium damage and IgG deposition was significantly higher than the control group, which is consistent with myocardial damage in patients with ward and myocardial deposition of patients with IgG, therefore, we believe that myocardial injury and selenium, food, water factors are closely related . Myocardial injury, leading to secondary immune pathology process.