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斯特朗尼柯娃等人收集了一些棉花黄萎病菌(Verticillium dahliae),这些病菌来自不同地区和不同的植株上。病菌纯培养后用30升发酵罐培养72小时,采用液体察氏培养基,按Grasset方法自分生孢子中获得水溶性抗原,然后进行抗原结构分析。结果证明,此菌有Ⅰ、Ⅱ血清型,中亚和摩尔达维亚地区的菌株以血清Ⅱ型为主。
Stronikova et al. Collected some Verticillium dahliae from different regions and different plants. After pure culture, the bacteria were cultured for 72 hours in a 30-liter fermentor. The water-soluble antigen was obtained from the conidia by the method of Grasset using the liquid culture medium, and then the antigen structure was analyzed. The results show that the bacteria have serogroups I, II, Central Asia and Moldavia strains of serum type Ⅱ.