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目的研究羽扇豆醇对人髓系K562、NB4、SHI-01及淋系Jurkat白血病细胞的增殖和凋亡作用。方法以不加药物处理为对照组,设置5个羽扇豆醇浓度梯度分别处理人髓系、淋系白血病细胞,采用MTT法检测细胞活力及增殖情况,流式细胞术检测细胞凋亡率。结果随着羽扇豆醇浓度的升高,细胞增殖明显受到抑制。当浓度达到150μmol/L时,4种细胞都呈现出明显的增殖抑制作用(均P<0.05);与对照组相比,羽扇豆醇组细胞凋亡率明显增加(P<0.05)。结论羽扇豆醇可显著抑制人髓系K562、NB4、SHI-01及淋系Jurkat白血病细胞增殖并诱导其凋亡。
Objective To study the effects of lupulonol on the proliferation and apoptosis of human myeloid line K562, NB4, SHI-01 and lymphocytic leukemia Jurkat cells. Methods Without drug treatment as control group, 5 lupine alcohol concentration gradients were set to treat human myeloid and lymphoid leukemia cells respectively. The cell viability and proliferation were detected by MTT assay. The apoptosis rate was detected by flow cytometry. Results As lupine alcohol concentration increased, cell proliferation was significantly inhibited. When the concentration reached 150μmol / L, all four kinds of cells showed obvious proliferation inhibition (all P <0.05). Compared with the control group, the apoptosis rate of lupuolol group increased significantly (P <0.05). Conclusion Lupeol can significantly inhibit the proliferation and induce the apoptosis of human myeloid line K562, NB4, SHI-01 and lymphocytic leukemia Jurkat cells.